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Korean J. Pl. Taxon > Volume 32(4); 2002 > Article
Korean Journal of Plant Taxonomy 2002;32(4): 383-396.
doi: https://doi.org/10.11110/kjpt.2002.32.4.383
RAPD에 의한 한국산 붓꽃속(Iris)의 계통분류학적 연구
박선주 , 심정기 , 박홍덕
A Molecular systematic study of Korean Iris (Iridaceae) based on RAPD analysis
Seon Joo Park, Jeong Ki Sim, Hong Duok Park
RAPD analyses were compared for 17 taxa of Korean Iris including the subgenus Sisyrinchium and Belamcanda. Eighty scorable RAPD markers were formed from the PCR reactions using 10 random oligoprimers. In this systematic analyses which used neighbor-joining methods including bootstrapping analyses with genetic coefficients, the Korean Iris were divided into three subgenera (Limniris, Crossiris, Pardanthopsis), or two genera (Limniris, Pardanthopsis). The molecular data agree with the previous classification system that recognized two sections and six series for the subgenus Limniris because the subgenus is comprised of four clades in the RAPD analyses. According to the molecula data, the series Chinensis should be divided into two groups. The minutoaurea group is composed of I. koreana, I. odaesanensis, and I. minitoaurea, while the rossi group is comprised of two varieties of I. rossi. The series Tripetalae is closely allied with the series Sibiricae, whereas the series Ensatae is recognized as a sister group to the series Ruthencae. The molecular phylogeny, which was based on RAPD analysis, for the most part agreed with the data proposed by previous authors. This is because the basis of morphological and ITS sequence data suggests that the RAPD markers should be very useful in addressing phylogenetic questions about the genus Iris
KeyWords: RAPD, NJ, Iris, RAPD, NJ, molecular, phylogeny
주요어: 붓꽃속, 계통학적 연구
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