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Korean J. Pl. Taxon > Volume 27(4); 1997 > Article
Korean Journal of Plant Taxonomy 1997;27(4): 415-428.
doi: https://doi.org/10.11110/kjpt.1997.27.4.415
RAPD 분석에 의한 한라산 자생 벚나무속 식물 및 재배 왕벚나무의 계통유전학적 유연관계
정용환, 고미희, 오유성, 김기옥, 정영철, 김문홍, 오문유
Phylogenetic Relationship Among Selected Taxa of Prunus in Mt. Halla and Cultivated Prunus yedoensis by the RAPD Analysis
Yong Hwan Jung, Mi Hee Ko, You Sung Oh, Gi Ok Kim, Young Cheul Chung, Moon Hong Kim, Moon You Oh
We analyzed the phylogenetic relationship among eight taxa (P pendula for. asendens Ohwi, P. yedoensis Matsumura, P sargentii Rehder, P. serrulata var. quelpaertensis Uyeki, P. maximowiczii Rupr., P. serrulata var. pubescens Nakai, P buergeriana Miquel, P. serrulata var. spontanea Wils.) of genus Prunus in Mt. Halla and a cultivated taxon, P. yedoensis Matsumura using random amplified polymorphic DNA(RAPD). RAPDs were used for identificaion on nine taxa by amplification using single 10-mer primers of arbitrary sequence. Nine taxa were clearly classified with eight arbitrary random primers which generated 76 polymorphic amplified DNAs or RAPDs. The phylogenetic tree was constructed from the RAPD fragment patterns by the neighbor-joining method. The genetic distance between Prunus sargentii Rehder and P. serrulata var. quelpaertensis Uyeki was 0.3036 and was the lowest among any other pairs. On the other hand, the genetic distance between P. yedoensis Matsumura and P. yedoensis cultivar was 0.4297. The molecular phylogeny based on RAPD analysis was considerably different from others based on morphology, palynology and isoenzymes. These results showed that RAPD analysis was a powerful tool for elucidating phylogenetic relationship among nine taxa of cherry.
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