| 엽록체 DNA를 이용한 섬괴불나무(Lonicera insularis Nakai)의 종내변이 및 지리학적 연구 |
| 정금선 , 김미선 , 이웅 , 박재홍 |
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| Intraspecific variation and geographic study of Lonicera insularis (Caprifoliaceae) based on chloroplast DNA sequences |
| Keum Seon Jeong, Mi Sun Kim, Woong Lee, Jae Hong Park |
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| Abstract |
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Lonicera insularis Nakai (Caprifoliaceae) is Korean endemic plant that lives along the shore of Ulleungdo and Dokdo. The aim of this study is to construct a phylogenetic relationship within six species (L.maackii, L.chrysantha, L.subsessilis, L. harai, L. morrowii) of genus Lonicera L. and Wigela subsessilis as outgroup and intraspecific variation of L. insularis using chloroplast DNA five regions sequences. Sequence analysis revealed that both L. insularis and L. morrowii showed complete homologies in the intergenic regions of trnL-trnF, trnS-trnG, psbM-trnD and matK coding region. However, sequence in the petN-psbM intergenic region showed a single nucleotide difference between both species, thus we designated them as CP01 and CP02. The plants having CP01 are prevalent in the Ulleungdo and Dokdo, while L. insularis and L. morrowii from Ulleungdo and of Dokdo, and Japan have CP02. This confirmed the existence of two cp DNA lineages with different geographical distributions. We can infer the allopatric speciation by geographical barrier. The result will provide the important basal data to study speciation and specie evolution of ocean islands such as Ulleungdo and Dokdo. |
| KeyWords:
Lonicera insularis, Ulleungdo, Dokdo, Chloroplast DNA, intraspecific variation |
| 주요어:
섬괴불나무, 울릉도, 독도, 엽록체 DNA, 종내변이 |
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