| Home | E-Submission | Sitemap | Editorial Office |  
top_img
Korean J. Pl. Taxon > Volume 41(4); 2011 > Article
Korean Journal of Plant Taxonomy 2011;41(4): 299-314.
doi: https://doi.org/10.11110/kjpt.2011.41.4.299
총설 : Microsatellite 개발 및 분석법에 대한 소개
윤영은 , 유정남 , 이병윤 , 곽명해
An Introduction to Microsatellite Development and Analysis
Young Eun Yun, Jeong Nam Yu, Byoung Yoon Lee, Myoung Hai Kwak
Received: October 3, 2011  Revised: November 4, 2011   Accepted: December 14, 2011
Abstract
The choice of molecular markers is the first step when selecting experimental plans in the field of population genetics. The popular molecular markers in population genetic studies are mainly allozyme, RAPD, RFLP, AFLP, microsatellite, SNP and ISSR. Among these, microsatellites are frequently found in nuclear, chloroplast and mitochondrial genome, showing a high level of polymorphism and nuclear microsatellites are codominant. Thus, it is a favorable molecular marker for population structure analyses and genetic diversity studies. Microsatellites are composed of tandem repeated 1~6 base pair nucleotide motifs and can be easily amplified by PCR reactions using locus specific primers. Because microsatellites have low cross-species transferability, however, they are only applicable between phylogenetically close species. In wild plants, the lack of genomic information and the high development cost of the microsatellite obstruct the wider use of microsatellites in plant population genetics research. In this review, we introduce the basis for microsatellite markers, the development process, and analytical methods as well as evolutionary models and their applications. In addition, possible genotyping errors which lead to erroneous conclusions are discussed.
KeyWords: Microsatellite, Genotyping, Population Genetics, Molecular Marker
주요어: 유전자형, 집단유전학, 분자마커
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
CrossRef TDM  CrossRef TDM
Share:      
METRICS
5
Crossref
0
Scopus
1,678
View
157
Download
Related article
Editorial Office
Korean Journal of Plant Taxonomy
Department of Biology, Daejeon University, Daejeon 34520, Korea
TEL: +82-42-280-2434   E-mail: kjpt1968@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © Korean Society of Plant Taxonomists.                 Developed in M2PI